: Using the computer software on your qPCR thermocycler, identify at which cycle the fluorescence intensity crosses a user‐specified threshold. Making use of good and damaging controls as benchmarks, select a threshold that Evidently distinguishes amplification from non‐amplification. This short article is remaining manufactured freely available by PubMed Central as https://www.quora.com/Can-nucleic-acid-extraction-be-automated-for-high-throughput-applications/answer/Even-Sa?prompt_topic_bio=1