Three plasmids (pGL1124, pGL1224 and pGL1217) were being built to enable the replacement of 1 allele of CYC9 Lister 427 cells. Trypanosomes were being transfected with each plasmid individually, and afterwards subjected to a second transfection with one of the other plasmids, with a special resistance gene, with all pairwise https://gastone433qbm5.iamthewiki.com/user